Fig 1: Decreased XPC expression promotes cell migration. (A). Cell scratch test to detect cell migration rate. All bar in the figure = 520 μm. (B). Use Image J to count the healing area of cell scratches. Cell migration rate = [(Sarea at 0 h–Sarea at 48 h)/Sarea at 0 h]×100%. The cell migration rate of the siXPC group was significantly higher than that of the siCtrl group (**p < 0.01, ***p < 0.001).
Fig 2: PSMD7 stabilizes RAD23B via deubiquitination in GC cells. (A) AGS cells were infected with lentiviruses carrying non-targeting (NT) shRNA or PSMD7 shRNA (shPSMD7-1 and shPSMD7-2) and analyzed by WB for RAD23B and XPC expression. (B) AGS cells were transfected with pcDNA3.1 vector carrying PSMD7 cDNA or an empty vector and analyzed by WB for RAD23B and XPC expression. (C) Co-IP assay confirmed the interaction between PSMD7 and RAD23B in AGS cells. (D) PSMD7 knockdown increased the ubiquitination of RAD23B in AGS cells. (E) AGS cells with or without PSMD7 silencing were treated with CHX (40 mg/mL) to block protein synthesis. WB was carried out to detect the RAD23B level 0, 1, 3, and 5 h after treatment. *P < 0.05.
Fig 3: XPC and CD133 Kaplan Meier curve. (A). Obtain the OS of 140 cases of lung adenocarcinoma from the HIS system, divide the expression of XPC in 140 cases of lung adenocarcinoma into two groups: XPC high expression and XPC low expression, and use Graphpad Primes 8 to analyze the survival of XPC in lung adenocarcinoma. Kaplan Meier curve found that, compared with the low XPC expression, the high XPC expression is related to the better prognosis of lung adenocarcinoma (Log-rank p = 0.0577). (B). Divide the expression of CD133 in 140 cases of lung adenocarcinoma into two groups: high expression of CD133 and low expression of CD133. Graphpad Primes 8 was used to analyze the survival of CD133 in lung adenocarcinoma. Kaplan Meier curve found that compared with the high expression of CD133, CD133 was low. The expression is related to the better prognosis of lung adenocarcinoma (Log-rank p = 0.00417).
Fig 4: The expression of XPC in lung adenocarcinoma cancer tissue and paracancerous tissue. (A). IHC detection of XPC expression in lung adenocarcinoma cancer tissue and paracancerous tissue. Brown means XPC positive reaction, XPC is expressed in the nucleus, and blue is nuclear staining. All bar in the figure = 50 μm. (B). GraphPad Primes 8 statistics found that XPC expression is higher in paracancerous tissue than in cancer tissue (p = 0.0344). (C). T is cancer tissue, P is paracancerous tissue. Western blot detects the expression of XPC in the fresh cancer tissue and paracancerous tissue of 8 lung adenocarcinoma patients. (D). Western blot counts the expression of XPC in fresh cancer tissue and paracancerous tissue of 8 lung adenocarcinoma patients, and the expression in paracancerous tissue is higher than that in cancer tissue (p < 0.0001). Bars represent the means ± SD of 8 lung adenocarcinoma patients.
Fig 5: Anti-tumor effect of XPC on A549 tumor-bearing nude mice. (A). Xenografts were generated by A549 cells (shCtrl), A549 cells with stable XPC knockdown (shXPC). Tumors were removed from mice after 32 days. (B). Tumor sizes were measured every 4 days. (C). The expression of CD133 and OCT4 were determined using Flow Cytometry. p < 0.0001(CD133), p < 0.0001(OCT4), A549-shXPC compared with A549-shCtrl. (D). The expression of XPC and CD133 were determined using Western blotting. The intensity of each band was quantified using Image J and normalized to. Protein extractions come from xenotransplanted tumor tissue of mice. ***p < 0.001 (XPC), *p < 0.05(CD133), A549-shXPC compared with A549-shCtrl. (n = 6).
Supplier Page from Abcam for Anti-XPC antibody - C-terminal